LSPR vs. SPR - Nicoya - Improving Human Life by Helping ...
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OpenSPR uses what is known as localized surface plasmon resonance (LSPR).Localized surface plasmon resonance is generated by metal nanoparticles, ... LSPRvs.SPRHowDoesSPRWork?LSPRvs.SPRSPReBookWhatisSurfacePlasmonResonance?WhySPR?Localizedsurfaceplasmonresonanceisgeneratedbymetalnanoparticles,typicallygoldorsilver,ascomparedtoacontinuousfilmofgoldasusedintraditionalSPR.OpenSPRissimilarinmanywaystotraditionalSPR,andprovidesthesamedatausingthesameexperimentalconditionsandprocedures.Totheuser,everythingwouldseemthesamebetweenotherSPRinstrumentsandOpenSPR.However,OpenSPRuseswhatisknownaslocalizedsurfaceplasmonresonance(LSPR).Localizedsurfaceplasmonresonanceisgeneratedbymetalnanoparticles,typicallygoldorsilver,ascomparedtoacontinuousfilmofgoldasusedintraditionalSPR.LSPRproducesastrongresonanceabsorbancepeakinthevisiblerangeoflight,withitspositionbeinghighlysensitivetothelocalrefractiveindexsurroundingtheparticle.Therefore,OpenSPRmeasuressmallchangesinthewavelengthoftheabsorbanceposition,ratherthanthetheangleoflightreflectedoffthegoldfilmasintraditionalSPR.Using LSPR insteadofSPRhasanumberofsignificantadvantages:TheopticalhardwareneededforLSPRismuchlesscomplexsincenoprismisneededtocouplethelight,sotheinstrumentcanbemadesmallerandmoreaffordableSincemeasuringareflectedlightangleisnotimportant,theinstrumentismuchmorerobustagainstvibrationandmechanicalnoiseLSPRisnotassensitivetobulkrefractiveindexchanges,whichcancauseerrorsintheexperimentaldataNostricttemperaturecontrolisneeded,simplifyingtheinstrumentThesensorchipscanbemanufacturedatamuchmoreaffordablepriceEasiertouseandmaintainAlthoughSPRsensorshaveamuchhigherrefractiveindexsensitivity(morRIS)thanLSPRsensors, theirsensitivitytowardsbiomolecularbindingeventsissimilar.Thisisduetothemuchshorterelectromagneticdecaylength(ld)ofnanoparticlescomparedtogoldfilms,whichconfinestheresponsetoasmallersensingvolume.AnLSPR’ssensorresponse(R)toanabsorbedlayercanbemodeledwiththefollowingequation:Where∆nisthedifferenceinrefractiveindexbetweentheabsorbedlayerandthesurroundingmedium,anddisthethicknessoftheabsorbedlayer.ThesmallerdecaylengthofSPRversusLSPRisillustratedintheimagebelow.ThissmallerconfinedsensingvolumemeansthatLSPRismoresensitivetomolecularbindingeventsatthesurface.ThesmallerdecaylengthandlowersensitivityassociatedwithLSPRreducesartifactscausedbyexternalvariablessuchastemperaturedriftorbufferrefractiveindexchanges.MakeyournextscientificbreakthroughwithSPRAreyoulookingtoobtainaffinityandkineticsdataforyournextpublication?Tohelpyougetstarted,we’vegatheredourexpertiseonthisinformation-richtechniqueintoahandyeBook.REQUESTEBOOKMeettheworld'sfirstdigitalhigh‑throughputbenchtopSPRsystem.AccelerateyourdrugdiscoverywithAlto.MeetAltoLaboratoryAutomationWebinarPopupLaboratoryAutomation FirstName*LastName*Email*SignUpforUpdatesYes!SendmegenuinelyusefulemailsfromNicoyaLifeScienceInc.everynowandthentohelpmestayuptodatewithSPR-relatedtopicsandNicoya’slatestnews.Seeourprivacypolicy,orcontactus.Youcanunsubscribeatanytime.Hiddenutm_campaignHiddenutm_contentHiddenutm_mediumHiddenutm_sourceHiddenutm_termHiddenformidHiddenwebinar-nameNameThisfieldisforvalidationpurposesandshouldbeleftunchanged.CLOSENavigatingthePost-CovidResearchLandscapePopupNavigatingthePost-CovidResearchLandscape FirstName*LastName*Email*SignUpforUpdatesYes!SendmegenuinelyusefulemailsfromNicoyaLifeScienceInc.everynowandthentohelpmestayuptodatewithSPR-relatedtopicsandNicoya’slatestnews.Seeourprivacypolicy,orcontactus.Youcanunsubscribeatanytime.Hiddenutm_campaignHiddenutm_contentHiddenutm_mediumHiddenutm_sourceHiddenutm_termHiddenformidHiddenwebinar-namePhoneThisfieldisforvalidationpurposesandshouldbeleftunchanged.CLOSECovid-19DiagnosticsUsingSPRPopupCovid-19DiagnosticsUsingSPR 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FirstName*LastName*Email*SignUpforUpdatesYes!SendmegenuinelyusefulemailsfromNicoyaLifeScienceInc.everynowandthentohelpmestayuptodatewithSPR-relatedtopicsandNicoya’slatestnews.Seeourprivacypolicy,orcontactus.Youcanunsubscribeatanytime.Hiddenutm_campaignHiddenutm_contentHiddenutm_mediumHiddenutm_sourceHiddenutm_termHiddenformidHiddenwebinar-nameEmailThisfieldisforvalidationpurposesandshouldbeleftunchanged.CLOSEPublishFasterWithBindingKinetics&AffinityDataOver600researchersworldwideareusingOpenSPR™togetthedatareviewersarelookingfor.Readourbrochuretolearnmore!"*"indicatesrequiredfieldsFirstName*LastName*Email*PhoneNumberSignUpforUpdatesYes!SendmegenuinelyusefulemailsfromNicoyaLifeScienceInc.everynowandthentohelpmestayuptodatewithSPR-relatedtopicsandNicoya’slatestnews.Seeourprivacypolicy,orcontactus.Youcanunsubscribeatanytime.Hiddenutm_campaignHiddenutm_sourceHiddenutm_mediumHiddenutm_contentHiddenutm_termHiddenformidPhoneThisfieldisforvalidationpurposesandshouldbeleftunchanged.CLOSEDownloadAltoBrochureThefutureofdrugdiscoveryisAlto.InterestedinlearningmoreabouthowAltocanaccelerateyourdrugdiscovery?Fillouttheformbelowtodownloadaproductbrochure."*"indicatesrequiredfieldsFirstName*LastName*Email*PhoneNumberSignUpforUpdatesYes!SendmegenuinelyusefulemailsfromNicoyaLifeScienceInc.everynowandthentohelpmestayuptodatewithSPR-relatedtopicsandNicoya’slatestnews.Seeourprivacypolicy,orcontactus.Youcanunsubscribeatanytime.Hiddenutm_campaignHiddenutm_sourceHiddenutm_mediumHiddenutm_contentHiddenutm_termHiddenformidPhoneThisfieldisforvalidationpurposesandshouldbeleftunchanged.CLOSE
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