利用低溫電漿化學改質鈦金屬表面 - 臺北醫學大學

論文名稱(英文), Chemical Modification of Titanium Surface by Glow Discharge ... 在膠樣金免疫標示觀察結果發現，經電漿表面處理再使用戊二醛交鏈劑作用，連接白 ... 進階搜尋 學院別 >臺北醫學大學 >口腔醫學院 >牙醫學系碩博士班   查詢北醫館藏 系統識別號 U0007-1704200714512816 論文名稱(中文) 利用低溫電漿化學改質鈦金屬表面 論文名稱(英文) ChemicalModificationofTitaniumSurfacebyGlowDischarge 校院名稱 臺北醫學大學 系所名稱(中) 牙醫學系碩博士班 系所名稱(英) GraduateSchoolofDentistry 學年度 91 學期 2 出版年 92 研究生(中文) 陳玫秀 研究生(英文) May-ShowChen 學號 M204090007 學位類別 碩士 語文別 中文 口試日期 論文頁數 69頁 口試委員 指導教授-李勝揚 中文關鍵字 鈦金屬  低溫電漿  交鏈劑  白蛋白  英文關鍵字 Titanium  glowdischarge  cross-linkingagent  albumin  學科別分類 中文摘要 良好生物相容性是植體的首要要求，而植體材料的本質及其表面特質決定其生物相容性，尤其是與宿主組織直接接觸的材料表面。

雖然目前對於植體表面與組織之間的反應、其長期穩定性及在臨床上的顯著性仍無定論，但已知植入後最早的生物反應為組織液蛋白質在植體表面的吸附，這層緊密吸附的蛋白質直接決定接下來宿主對植體表面的細胞反應，所以為了提昇植體與組織間整合，可藉由控制界面反應，吸附特定蛋白質，進而導引有利之組織癒合。

因此本研究利用低溫電漿來活化鈦金屬表面以進一步連接白蛋白，企圖改變鈦金屬表面化學性質，發展一種能鍵結已知生物活性分子到鈦金屬表面的技術。

鈦金屬圓片先以氬氣電漿去除表面污染物，來產生可重複取得的清潔表面，接著用丙烯胺電漿處理，使丙烯胺聚合在鈦金屬表面，再以交鏈劑戊二醛將白蛋白與丙烯胺的胺基(-NH2)鍵結。

樣本以掃描式電子顯微鏡(SEM)及原子力顯微鏡﹙AFM﹚觀察表面形態變化，以能量分散光譜儀(SEM-EDS)進行表面元素變化之定性分析，使用X光光電子能譜儀(XPS)檢測表面化學鍵結與元素變化作定性分析與半定量分析，並以膠樣金免疫標示法(colloidalgoldimmunolabeling)配合使用場發射鎗掃描式電子顯微鏡(FieldEmissionScanningElectronMicroscope,FEGSEM)，觀察鈦金屬表面白蛋白連接情形作定性分析與定量分析。

經SEM及AFM觀察得知，氬氣電漿處理的時間與表面潔淨度及表面粗糙度有正相關，此結果顯示經電漿解離的氬離子與原子具清潔及離子轟擊(ionbombardment)鈦金屬表面的功能；丙烯胺的聚合厚度隨著丙烯胺電漿作用時間的增加而增加，另外鈦金屬表面形態除了氬氣電漿處理組與丙烯胺電漿處理組相似外，其他組別也隨著處理程序展現不同變化。

SEM-EDS的結果顯示在未經任何處理及經氬氣電漿清潔後的鈦金屬表面，偵測到主要為鈦元素，丙烯胺電漿處理後，即可測到氮元素；戊二醛作用後表面有氧元素的出現；白蛋白處理後即可測得碳及氧元素。

XPS更進一步證實，氬氣電漿只有清潔鈦金屬表面的作用，並不會改變其表面化學組成；其餘各組的鈦、碳、氮、氧的比例及鍵結能量隨著處理程序而改變，可知表面上有化學組成之改變，且隨不同處理程序所得到表面元素之百分比及鍵結能量，與預期的表面披覆物元素百分比及鍵結能量相近。

在膠樣金免疫標示觀察結果發現，經電漿表面處理再使用戊二醛交鏈劑作用，連接白蛋白到鈦金屬表面的密度，已多於預期可引發生物活動的數目。

因此使用電漿處理技術可達到鈦金屬表面胺基化，加上交鏈劑戊二醛的處理後，可使鈦金屬表面接上白蛋白產生表面改質，確可提供一個鍵結已知生物活性分子到鈦金屬表面的方式，以期植體植入後能引導及促進組織復原。

英文摘要 Biocompatibilityistheprimerequisiteforimplantmaterialandisdeterminedbythebulkpropertiesandespeciallythesurfaceofimplantwhichdirectlycontactingthehosttissue.Eventhoughtherelationshipsandreactionsbetweenthesurfaceofimplantandtissueandtheirlong-termintegrityandclinicalefficacyarestillnotwellunderstood,thefirstbiologicalreactionknowntooccurafterimplantationofabiomaterialistheadsorptionoftissuefluidproteinsontoitssurfaceandthistightlyproteinsstronglyinfluencessubsequentinteractionsofcellswiththesurface.Inordertooptimizetheintegrationofimplants,itisdesirabletocontrolinterfacialreactionssuchthatnonspecificadsorptionofproteinsisminimizedandbeneficialmoleculesareselectivelyadsorbedontobiomaterialspriortotheirimplantation.Inthisregard,ourgoalistodevelopaglow-dischargemethodtofunctionalizetitaniumsurfacesbythecovalentimmobilizationofbioactiveorganicmolecules.TitaniumplatefirstwascleanedbyglowDischargeusingargonplasmatoeliminatesurfacecontaminantsandtoproduceaconsistentandreproducibletitaniumoxidesurfacelayer.Thenanintermediaryallylaminedepositionwascovalentlylinkedtotheoxidelayerbyglowdischarge,followedbythecovalentbindingofalbumintothefreeterminalNH2groupsusingglutaraldehydeasacouplingagent.Surfacemorphologieswereobservedbyscanningelectronmicroscopy(SEM)andatomicforcemicroscopy(AFM).Analysesofsurfacecompositionwereperformedbyscanningelectronmicroscopyenergydispersivespectroscopy（SEM-EDS)andX-rayphotoelectronspectroscopy(XPS),andSurfacecoveragebyboundalbuminwasevaluatedbyfieldemissionscanningmicroscope(FEGSEM)visualizationofcolloidalgoldimmunolabeling.TheSEMandAFMresultshowedthatthesurfacecontaminantsandroughnessweredecreasedasthetreatingtimeofArgonplasmaincreased.Thedepositionthicknessofallylaminewasincreasedwiththetreatingtime.Thesurfacetextureswerechangedfollowingdifferentproceduresexceptallylaminegroupsanditmeansthatthe30mindepositionthicknessofallylamineistoothintochangethesurfacecontours.TheSEM-EDSshowedthatthecontentofsurfaceelementswaschangedaftertreatedwithglutaraldehydeoralbuminindicatedthatargonplasmaonlyhadsurfacecleaningeffectanddidnotchangesurfacecompositionandtheallylaminedepositionthicknesswastoothintoshowthedifferenceinSEM-EDSfindings.TheXPSresultsconfirmedtheargonplasmacleaningeffectandrevealedthattheratioandthebindingenergyofsurfaceelementschangedwithdifferenttreatmentproceduresandweresimilartowhatexpectedtocoatonthetitaniumsurface.Thebindingdensityofimmunolabelingforalbuminsuggeststhatthesurfacecoverageofalbuminisinexcessofwhatexpectedforinducingbiologicalactivity.Sothesurfacecharacteristicswassuccessfulmodifiedonthetitaniumplatesbyglowdischargetechnologyandthismethodcouldofferthepossibilityofcovalentlylinkingselectedmoleculesinordertoguideandpromotethetissuehealingthatoccursduringimplantintegrationinboneandsofttissue. 論文目次 致謝Ⅰ中文摘要Ⅱ英文摘要Ⅳ目錄Ⅵ圖表目次Ⅷ第一章緒論1第一節研究動機與重要性1第二節研究目的2第三節研究假設…………………………………………………………………………..2第四節名詞界定2第二章文獻查證5第一節植體材料發展背景5第二節鈦金屬6第三節鈦金屬的表面改質8第四節低溫電漿表面處理12第五節氬氣電漿15第六節丙烯胺電漿16第七節戊二醛交鏈劑16第八節白蛋白17第三章研究材料與方法……………………………………………………………………...18第一節研究材料………………………………………………..…………………………18第二節研究儀器…………………………………………………………………………..19第三節研究步驟與方法…………………………………………………………………19第四章研究結果……………………………………………………………………………..24第一節鈦金屬表面形態觀察結果…………………………………………………….24第二節鈦金屬表面化學分析結果…………………………………………………….25第五章討論………………………………………………………………………………………29第六章結論與建議…………………………………………………………………………….33第一節結論…………………………………………………………………………………..33第二節研究限制……………………………………………………………………………33第三節應用與建議…………………………………………………………………………34參考資料……………………………………………………………………………………………..35 參考文獻 1.B.D.Ratner,“Advancesintheanalysisofsurfacesofbiomedicalinterest”,Surf.Interf.Anal.,23,521-528(1995).I2.J.Lausmaa,B.Kasemo,U.Rolander,L.M.Bjursten,L.E.Ericson,L.Rosander,andP.Thomsen,“Preparation,surfacespectroscopicandelectronmicroscopiccharacterizationoftitaniumimplantmaterials”,inSurfacecharacterizationofbiomaterials,B.D.Ratner(ed.),ElsevierSciencePublishers,Amsterdam,1988,pp.161-174.3.A.F.vonRecumandT.G.vanKooten,“Theinfluenceofmicro-topographyoncellularresponseandtheimplicationsforsiliconeimplants”,JournalofBiomaterialsSciencePolymer,7,181-198(1995).4.S.A.Rowland,S.W.Shalaby,R.A.Latour,andA.F.Jr.&vonRecum,“Effectivenessofcleaningsurgicalimplants:quantitativeanalysisofcontaminantremoval”,JournalofAppliedBiomaterials,6,1-7(1995).5.S.G.Steinemann,“Titanium-thematerialofchoice?Periodontology2000,17,7-12(1998).6.C.A.vanBlitterswijk,D.Bakker,S.C.Hesseling,andH.K.Koerten,“Reactionsofcellsatimplantsurfaces”,Biomaterials,12,187-193(1991).7.P.Tengvall,I.Lundstrőm,L.Sjőqvist,H.Elwing,andL.M.Bjursten,“Titanium-hydrogenperoxideinteraction:Modelstudiesoftheinfluenceoftheinflammatoryresponseontitaniumimplants”,Biomaterials,10,166-175(1989).8.J.H.Doundoulakis,“Surfaceanalysisoftitaniumaftersterilization:Roleinimplant-tissueinterfaceandbioadhesion”,J.Prosthet.Dent.,58,471-478(1987).9.R.E.Baiser,A.E.Meyer,C.K.Akers,J.R.Natiella,M.Meenaghan,andJ.M.Carter,“Degradationeffectsofconventionalsteamsterilizationonbiomaterialsurfaces”,Biomaterials,3,241-245(1982).10.N.L.HernándezdeGatica,G.L.Jones,andJ.A.J.Gardella,“Surfacecharacterizationoftitaniumalloyssterilizedforbiomedicalapplications”,Appl.Surf.Sci.,68,107-121(1993).11.B.D.Ratner,“Newideasinbiomaterialsscience-APathtoengineeredbiomaterials”,J.Biomed.Mater.Res.,27,837-850(1993).12.T.A.Horbett,“Principlesunderlyingtheroleofadsorbedplasmaproteinsinbloodinteractionswithforeignmaterials”,Cardivasc.Pathol.,2,137S-148S(1993).13.L.TangandJ.W.Eaton,“Fibrin(ogen)mediatesacuteinflammatoryresponsestobiomaterials”,J.Exp.Med.,178,2147-2156(1993).14.B.Wälivaara,B.O.Aronsson,M.Rodahl,J.Lausmaa,andP.Tengvall,“Titaniumwithdifferentoxides:Invitrostudiesofproteinadsorptionandcontactactivation”,Biomaterials,15,827-834(1994).15.D.F.Williams,“Tissue-materialinteractions”,J.Mat.Science,22,3421-3444(1987).16.T.A.Horbbet,“Proteinadsorptiononbiomaterials”,in:S.L.Cooper,N.A.Peppas,Biomaterials:interfacephenomenaandapplications,WashingtonDC:AmericanChemicalSociety,233-244(1982).17.J.E.Davies,“Theimportanceandmeasurementofsurfacechargespeciesincellbehavioratthebiomaterialinterface”,in:B.D.Ratner,SurfaceCharacterizationofBiomaterials,Amsterdam;Elsevier,219-222(1988)18.K.C.DeeandR.Bizios,“Mini-review:Proactivebiomaterialsandbonetissueengineering”,Biotechnol.Bioeng.,50,438-442(1996).19.陳錦煜碩士論文，”利用低溫電漿處理鈦金屬表面之蛋白質固定”,台北醫學大學口腔復健醫學研究所,(2002).20.J.E.LemonsandF.Dietsh-Misch,“BiomaterialsforDentalImplants”,in:C.E.Misch,ContemporaryImplantDentistry,Mosby,2nded.,271-302(1999).21.BranemarkPI.BreineU.AdellR.HanssonBO.Intraosseousanchorageofdentalprostheses.IExperimentalstudies.ScandJPlastReconstrSurg.3:81,1969.22.SchroederA.PohlerO.SutterF.GewebsreaktionaufeinTitan-Hohlzylinderim-plantatmitTitan-SpritzschichtoberflacheSchweizMonatsschrZahnheilk.86:713,1976.23.BranemarkPI.HanssonBO.AdellR.BreineU.LindstromJ.HallenO.OmannA.Osseointegratedimplantsinthetreatmentoftheedentulousjaw.Experiencefroma10-yearperiod.ScandJPlastReconstrSurg.11(suppl),1977.24.SchroederA.StichH.StraumannF.SutterF.UberdieAnlagerungvonOsteozementaneinenbelastetenImplantatkorper.SchweizMonatsschrZahnheilk.88:1051,1978.25.SchroederA.VanderZypenE.StichH.SutterF.Thereactionofbone,connectivetissueandepitheliumtoendostealimplantswithsprayedtitaniumsurface.JMaxillofacSurg.9:15,1981.26.P.I.Brånemark,“Introductiontoosseointegration”,in:Tissue-IntegratedProstheses,OsseointegrationinClinicalDentistry,eds.P.I.Brånemark,G.ZarbandT.Albrektsson,Chicago,Berlin:QuintessencePublishingCo,1985.27.L.SennerbyandJ.Roos,“Surgicaldeterminantsofclinicalsuccessofosseointegratedoralimplants:Areviewoftheliterature”,TheInternationalJ.Prosthodontics,11,408-418(1998).28.T.Albrektsson,P.I.Brånemark,H.A.HanssonandJ.Lindstrőm,“Osseointegratedtitaniumimplants”ActaOrthopedicaScandinavica,52,155-170(1981).29.K.A.ThomasandS.Cook,“Anevaluationofvariablesinfluencingimplantfixationbydirectboneapposition”,J.Biomed.Mat.Res.,19,875-901(1985).30.M.Espositoetal.,“Biologicalfactorscontributingtofailuresofosseointegratedoralimplants(II).Etiopathogenesis”,Eur.J.OralSci.106,721-764(1998).31.賴耿陽編著,“金屬鈦”,復漢出版社,第一章金屬鈦的資源與製煉,1989.32.KasemoB.Biocompatibilityoftitaniumimplants:Surfacescienceaspects.JProsthetDent,49:832,1983.33.AhmadM.GawronskiD.BlumJ.GoldbergJ.GronowiczG.Differentialresponseofhumanosteoblast-likecellstocommerciallypure(cp)titaniumgrades1and4.JournalofBiomedicalMaterialsResearch.46(1):121-31,1999.34.BrånemarkP-I.,RydevikBjörnL.,SkalakRichard.Osseointegrationinskeletalreconstructureandjointreplacement,secondinternationalworkshoponosseointegrationinskeletalreconstructionandjointreplacement.RanchoSantaFE,California,October27-29,1994.chapter2,History,Development,andCurrentStatusofOsseointegrationasRevealedbyExperienceinCraniomaxillofacialsurgery.PhilipWorthington.25-44.35.LautenschlagerE.P.andMonaghanP.,“Titaniumandtitaniumalloysasdentalmaterials”,InternationalDentalJournal,43:245-253,1993.36.J.Meyle,“Celladhesionandspreadingondifferentimplantsurfaces”,in:N.P.Lang,T.KarringandJ.Lindhe,Proceedingsofthe3rdEuropeanWorkshoponPeriodontology,ImplantDentistry,EuropeanAcademyofPeriodontology,55-72(1999).37.J.E.Ellingsen,“Surfaceconfigurationsofdentalimplants”,Periodontology2000,17,36-46(1998).38.U.Grossetal.,“Implantsurfaceroughnessandmodeofloadtransmissioninfluenceperiimplantbonestructure”,Adv.Biomater.9,303-309(1990).39.R.Baieretal.,“Surfacepropertiesdeterminebioadhesiveoutcomes”,J.Biomed.Mat.Res.18,337-355(1984).40.R.E.BaierandA.E.Meyer,“Implantsurfacepreparation”,Int.J.Oral.Maxillofac.Impl.,3,3-19(1988).41.W.A.Lacefield,“materialscharacteristicsofuncoated/ceramic-coatedimplantmaterials”,Adv.Dent.Res.,13,21-26(1999).42.B.KasemoandJ.Gold,“Implantsurfacesandinterfaceprocesses”,Adv.Dent.Res.,13,8-20(1999).43.Y.Itoetal.,“Cellgrowthonimmobilizedcell-growthfactor.II.Adhesionandgrowthoffibroblastcellsonpoly(methylmetharylate)membraneimmobilizedwithproteinsofvariuskinds”,Biomaterials,13,789-794(1992).44.Y.Itoetal.,“Cellgrowthonimmobilizedcell-growthfactor.IV.Enhancementoffibroblastcellgrowthbyimmobilizedinsulinand/orfibronectin”,J.Biomed.Mater.Res.,27,901-907(1993).45.S.Q.Lin,Y.Ito,andY.Imanishi,“Cellgrowthonimmobilizedcellgrowthfactor.IX.Covalentimmobilizationofinsulin,transferring,andcollagentoenhancegrowthofbovineendothelialcells”,J.Biomed.Mater.Res.,27,909-915(1993).46.Y.Ito,S.Q.Liu,Y.Imanishi,“Enhancementofcellgrowthongrowthfactor-immobilizedpolymerfilm”,Biomaterials,2,449-453(1991).47.A.Kishidaetal.,“Immobilizationofhumanthrobomodulinontopoly(etherurethaneurea)fordevelopingantithrobogenicblood-contactingmaterials”,Biomaterials,15,848-852(1994).48.A.Kishidaetal.,“Immobilizationofhumanthrobomodulinonbiomaterials:Evaluationoftheactivityofimmobilizedhumanthrobomodulin”,Biomaterials,15,1170-1174(1994).49.M.Ticháetal.,“Immobilizationofheparinonpolyacrylamidederivatives”,J.Chromatogr,656B,423-426(1994).50.S.E.Lynchetal.,“Effectsoftheplatelet-derivedgrowthfactor/insulin-likegrowthfactorIcombinationonboneregenerationaroundtitaniumdentalimplants,resultsofapilotstudyinbeagledogs”,J.Periodontol.,62,710-716,1991.51.T.Kawaietal.,“Osteoinductiveactivityofcompositesofbonemorphogeneticproteinandpuretitanium”,Clin.Orthop.,290,296-305,1993.52.H.Schliephakeetal.,“EffectofRGDpeptidecoatingoftitaniumimplantsonperiimplantboneformationinthealveolarcrest”,Clin.OralImpl.Res.,13,312-319(2002).53.R.E.Maxetal.,“Platelet-richplasma:growthfactorenhancementforbonegrafts”,OralSurg.OralMed.OralPathol.,85,638-646,1998.54.E.Anitua,“Plasmarichingrowthfactors:preliminaryresultsofuseinthepresentationoffuturesitesforimplants”,Int.J.OralMaxillofac.Implants,14,529-533,1999.55.R.E.Max,“Platelet-RichPlasma﹙PRP﹚:WhatisPRPandwhatisnotPRP?”,ImplantDentistry,10,225-228,2001.56.D.A.Puleo,“BiochemicalsurfacemodificationofCo-Cr-Mo”,Biomatrerials,17,217-222(1996).57.A.Nacietal.,“Chemicalmodificationoftitaniumsurfacesforcovalentattachmentofbiologicalmolecules”,JBiomed.Mater.Res.,40(2),324-335(1998).58.C.D.BainandS.D.Evans,“Layingitonthin”,Chem.Br.,31,46-48,1995.59.M.MrksichandG.M.Whitesides,“Usingself-asssembledmonolayerstounderstandtheinteractionsofman-madesurfaceswithproteinsandcells”,Ann.Rev.Biophys.Biomol.Struct.,25,55-78,1996.60.Ih-HoungLoh,ScD,“Plasmasurfacemodificationinbiomedicalapplications’’AdvancedSurfaceTechnologyInc.,Billerica,Massachusetts.MedicalDevicetechnology.Jan/Febissues(1999).61.P.K.Chuetal.,“Plasma-surfacemodificationofbiomaterials”,Mater.Sci.Eng.R.,36,143-206(2002).62.Boenig,H.V.Plasmascienceandtechnology.pp.16-35.CornellUniversityPress.1982.63.B.Chapman,GlowDischargeProcesses—SputteringandPlasmaEtching,NewYork,Wileypp.106-109,1980.64.A.Grill,Coldplasmainmaterialsfabrication:fromfundamentalstoapplications.pp.151-179.IEEEPress.1994.65.陳克紹。

低溫電漿表面處理技術原理概述與應用簡介。

低溫電漿表面處理技術於高分子材料上之應用研討會。

於新竹市工研院化工所。

5:29-30,1997.66.S.Conroyetal.,”Plasmacleaningofmedicaldevices”,A2C2,TheJournalofAdvancingApplicationsinContaminationControl,Vol.3,No.6,June2000.67.R.E.BaierandV.A.DePalma,ElectrodelessGlowDischargeCleaningandActivationofHigh-EnergySubstratestoInsureTheirFreedomfromOrganicContaminationandTheirReceptivityforAdhesivesandCoatings.Buffalo,NY:CornellAeronauticalLaboratory,1970:176.68.K.M.Swartetal.,“Short-termplasma-cleaningtreatmentsenhanceinvitroosteoblastattachmenttotitanium”,JOralImplantol.,18,130-137,1992.69.B.-O.Aronssonetal.,“Glowdischargeplasmatreatmentforsurfacecleaningandmodificationofmetallicbiomaterials”,J.Biomed.Mater.Res.35,49-73(1997).70.Y.ShibataandS.Fujimori,“Glowdischargetreatmentiseffectiveforcellproliferationanddifferentiationofosteoblasts-likecellsontitaniumsurface”,JShowaUnivDentSoc,20,24-29,2000.71.Y.ShibataandT.Miyazaki,“Anodeglowdischargeplasmatreatmentenhancescalciumphosphateadsorptionontotitaniumplates”,JournalofDentalResearch,81,841-847,2002.72.Y.Shibataetal.,“Glowdischargeplasmatreatmentoftitaniumplatesenhancesadhesionofosteoblast-likecellstotheplatesthroughtheintegrin-mediatedmechanism”,IntJOralMaxillofacimplants,17,771-777,2002.73.JoseG.Calderonetal.,“Stabilityofplasma-polymerizedallyaminefilmswithsterilizationbyautoclaving”,JBiomedMaterRes.,42,597-603(1998).74.TimothyJ.A.Johnson.Eur.J.CellBiol.45:160-169,1987.75.K.G.Draperetal.,BiochemicalandBiophysicalresearchcommunications,163:1,466-470,1989.76.Y.Itoetal.,“Materialsforenhancingcelladhesionbyimmobilizationofcell-adhesivepeptide”,J.Biomed.Mater.Res.,25,1325-1337,1991.77.M.Takatsukaetal.,“PreparationofRGD-Albuminconjutes:analysisofcellularadhesivefunctionsinvitro”,Jpn.J.Artif.Organs,21:1,191-195,1992.78.Y.H.Anetal.,“Rapidquantificationofstaphylococciadheredtotitaniumsurfacesusingimageanalyzedepifluorescencemicroscopy”,Trans.Soc.Biomat.,16,36,1993.79.S.G.McDowelletal.,“Applicationofafluorescentredoxdyeforenumerationofmetabolicallyactivebacteriaonalbumin-coatedtitaniumsurfaces”,Lett..App.Microbiol.,21,1-4,1995.80.Y.H.Anetal.,“Preventionofbacterialadherencetoimplantsurfacewithacrosslinkedalbumincoatinginvitro”,J.Orth.Res.,14:5,846-849,1996.81.T.M.S.Chang,“Platelet-surfaceinteraction:effectofalbumincoatingorheparincomplexingonthrombogenicsurfaces”,Can.J.Physiol.Pharmacol.,52,275-285,1974.82.A.RemuzziandP.Boccardo,“AlbumintreatmentreducesinvitroplateletdepositiontoPMMAdialysismembrane”,Int.J.Artif.Organs”,16,128-131,1993.83.M.Bendayan,“Colloidalgoldpost-embeddingimmunocytochemistry”,Prog.Histochem.Cytochem.,29,1-163,1995.84.R.W.PaynterandB.Ratner,“ThestudyofinterfacialproteinsandbiomoleculesbyX-rayphotoelectronspectroscope”,InSurfaceandinterfacialAspectsofBiomedicalPolymers,Vol.2,ed.J.D.Andrade,PlenumPress,NewYork,1985,pp.189-216.85.K.S.RobinsonandP.M.A.Sherwood,“X-rayphotoelectronspectroscopicstudiesofthesurfaceofsputterionplatedfilms”,Surf.Interf.Anal.,6,261-266,1984.86.B.D.Ratner,“Characterizationofbiomaterialsurfaces”,Cardiovasc.Pathol.,2(Suppl.),87-100,1993.87.R.P.Singhetal.,“DefinitionofaspecificinteractionbetweentheearlyTlymphocyteactivation1(Eta-1)proteinandmurinemacrophagesinvitroanditseffectuponmacrophagesinvivo”,J.Exp.Med.,171,1931-1942,1990. 論文全文使用權限 不同意授權瀏覽/列印電子全文服務。

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