Net Protein Utilization Determined in Short- and Long-term ...

Abstract. The protein quality, measured as net protein utilization, of casein (10.5 protein calories per cent total calories in the diet) and gluten (35.6 ... SkiptoMainContent Advertisement SearchMenu Menu NavbarSearchFilter ThisissueAllTheJournalofNutrition AllASNJournalsAllJournals MobileMicrositeSearchTerm Search SignIn Issues MoreContent AdvanceArticles Supplements ASNJournals ResearchNeeds FoodEnvironmentandSupplyonDietaryandLifestyleChoices Growth,Development,andReproduction NutritioninHealthMaintenance NutritioninMedicalManagement Nutrition-relatedBehaviors VariabilityinDietandFoodResponses BrowseAll Submit AuthorGuidelines SubmissionSite CallsforPapers AnnualAvianResearchAward OpenAccessOptions Self-ArchivingPolicy Purchase About AboutTheJournalofNutrition AboutTheAmericanSocietyforNutrition EditorialBoard ReviewerRecognition Permissions Alerts OtherPolicies Advertising&CorporateServices JournalsCareerNetwork Issues MoreContent AdvanceArticles Supplements ASNJournals ResearchNeeds FoodEnvironmentandSupplyonDietaryandLifestyleChoices Growth,Development,andReproduction NutritioninHealthMaintenance NutritioninMedicalManagement Nutrition-relatedBehaviors VariabilityinDietandFoodResponses BrowseAll Submit AuthorGuidelines SubmissionSite CallsforPapers AnnualAvianResearchAward OpenAccessOptions Self-ArchivingPolicy Purchase About AboutTheJournalofNutrition AboutTheAmericanSocietyforNutrition EditorialBoard ReviewerRecognition Permissions Alerts OtherPolicies Advertising&CorporateServices JournalsCareerNetwork Close searchfilter Thisissue AllTheJournalofNutrition AllASNJournals AllJournals searchinput Search AdvancedSearch SearchMenu ArticleNavigation Closemobilesearchnavigation ArticleNavigation Volume87 Issue2 October1965 ArticleNavigation ArticleNavigation NetProteinUtilizationDeterminedinShort-andLong-termExperimentswithRats Getaccess MariaAngelicaTagle, MariaAngelicaTagle 8LaboratoryofNutrition,SchoolofPublicHealth,UniversityofChile,Santiago,Chile Searchforotherworksbythisauthoron: OxfordAcademic GoogleScholar GonzaloDonoso GonzaloDonoso 8LaboratoryofNutrition,SchoolofPublicHealth,UniversityofChile,Santiago,Chile Searchforotherworksbythisauthoron: OxfordAcademic GoogleScholar TheJournalofNutrition,Volume87,Issue2,October1965,Pages173–178,https://doi.org/10.1093/jn/87.2.173 Published: 01October1965 Articlehistory Received: 09February1965 Published: 01October1965 Views Articlecontents Figures&tables Video Audio SupplementaryData Cite Cite MariaAngelicaTagle,GonzaloDonoso,NetProteinUtilizationDeterminedinShort-andLong-termExperimentswithRats,TheJournalofNutrition,Volume87,Issue2,October1965,Pages173–178,https://doi.org/10.1093/jn/87.2.173 SelectFormat Selectformat .ris(Mendeley,Papers,Zotero) .enw(EndNote) .bibtex(BibTex) .txt(Medlars,RefWorks) Downloadcitation Close PermissionsIcon Permissions Share Email Twitter Facebook More NavbarSearchFilter ThisissueAllTheJournalofNutrition AllASNJournalsAllJournals MobileMicrositeSearchTerm Search SignIn Close searchfilter Thisissue AllTheJournalofNutrition AllASNJournals AllJournals searchinput Search AdvancedSearch SearchMenu Abstract Theproteinquality,measuredasnetproteinutilization,ofcasein(10.5proteincaloriespercenttotalcaloriesinthediet)andgluten(35.6proteincaloriespercenttotalcaloriesinthediet),wasevaluatedbymeansofshort-and,long-termexperiments.Caseinandglutendietsofequalproteinvalue(asnetdietaryproteincaloriespercent)werechosenasrepresentativeoftheaminoacidlimitationsmostfrequentlyobservedinhumannutrition:sulphur-bearingaminoacidsandlysine.Fortheshort-termexperiment,thenetproteinutilizationwasdeterminedbythemethodofMillerandBenderon31-day-oldrats.Forthelong-termexperiment,ratswerefedthedietsunderstudyfromthetimetheywereweaneduntiltheywere133daysold.Thenetproteinutilizationwascalculatedonthebasisofbodynitrogengain,lossofendogenousnitrogenandnitrogenintake,accordingtoaformulaproposedbytheauthors.Thenetproteinutilizationvaluesforbothdietsdeterminedbytheshort-termexperiment(70.7and19.8,respectively,forthecaseinandglutendiets)areinagreementwiththosecalculatedforthelong-termexperiment(75.2and19.8).Theproteinvalue,measuredasnetdietaryproteincalories,ofcaseinandglutendietsobtainedfroma10-dayexperimentisinagreementwiththatobtainedfroma112-dayexperiment. 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